Print Page - Filters for sample clean-up

Title: Filters for sample clean-up
Post by: prao on April 03, 2012, 03:29:03 PM

Hello,
I am kind of getting in to the metabolomics area from Proteomics and wanted to know if there are any suggestions as to sample cleanup after deproteinizing the serum with organic solvents. I have used C18 for peptides but using that for metabolites seems to be biased for hydrophobic ones. Are there any low binding filter materials that can be used. Also, I do not know if sample cleanup will help in enhancing the intensities of metabolites using 100% methanol for extraction. I am getting extremely weak signal intensities. Instrument is a Thermo LTQ. Any suggestions for cleanup would be highly apreciated.

Thank you

Title: Re: Filters for sample clean-up
Post by: hpbenton on April 04, 2012, 08:12:40 PM

Prao,

If I understand correctly you're really looking for an optimum method. The following are worth looking at:

Quote

(1) Geier, F. M.; Want, E. J.; Leroi, A. M.; Bundy, J. G. Anal. Chem. 2011, 83, 3730–3736.

Hope it's what you're thinking and is helpful.

Paul

Title: Re: Filters for sample clean-up
Post by: prao on April 05, 2012, 08:38:32 AM

Thanks Paul for the prompt reply. I tried these methods of extraction but my problem was that the column clogged in the nano-spray mode and I need to clean-up my sample using some kind of non-biased filter which will help filter out particulates produced during the preparation of the sample. Please let me know if you have any suggestions. I have used the centrifugal method but the column still clogs up.

Thanks
Prao

Title: Re: Filters for sample clean-up
Post by: edward745 on October 12, 2014, 11:36:29 PM

good keep it up

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Scientific themes => Analytical techniques => Sample preparation => Topic started by: prao on April 03, 2012, 03:29:03 PM