profile vs centroid, file size issue October 11, 2013, 04:35:11 AM HI, I am using the Agilent Q-TOF LC/MS with MassHunter software to acquire my sample signal. To use the XCMS online, the large size of original file is a bit daunting and troublesome. So I want to ask about the acquisition mode and file format issue. Should I acquire in default profile mode and then export/convert in MassHunter to mzDATA format (or other formats), which results in much smaller size? Do I need to set a reasonable Peakfilter?Or should I aqcuire diectly in centroid mode, which can also give me a smaller file?Does anyone have experience with this issue, which way can better preserve the signals for downstream analyses? Thanks in advance for suggestions.Lingyun Quote Selected