I am working on plant Arabidopsis. I want to quantify suberin monomers in Arabidopsis stem by using HPLC-MS (LC-ESI-LTQOrbitrap).For this I am extracting samples using 60% methanol and will be using negative ionization. is that ok.. or do I need to take care in any steps to identify suberin monomers...
Hi, I have problem with retention time deviation.The instrument I am using is HPLC LTQ-orbitrab. my samples are deviating upto 0.5 min(rt deviation file attached) for this I have set a parameter bw=30sec(allowable RT correction) .so is this RT deviation of samples is acceptable or to reduce do I need to change parameter for RT correction?,if so what value shall I give. other parameters that I have set are as follows: 1.negative ionisation 2.centwave method 3.ppm:20 (maximal tolerated m/z deviation in consecutive scans, in ppm (parts per million) 4.min peak width:10 5.max peak width:80 6.s/n threshold:5 7.integration method:1 prefilter peaks:3 9.peak intensity:2500 10.noise filter:2500 11.rt correction method:obiwrap 12.profstep:1 13.bw=30 14.mzwid:0.015 15.minfrac:0.5 16.max:100 17.minsample:3 18.isotopes and adducts 19.5ppm 20.for identification 5 ppm and for adducts M-H please let me know the set parameters are correct or do i need to change any of them. thank you .
