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Messages - 7clouds7
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
I tried using proteowizard and just only focusing on data from 50-500 seconds. But unfortunately, I am still seeing the high %CV's. This is a bummer. I have included the picture of the proteowizard parameters and also some of the amino acids that normally had low %CV's (phenylalanine and glutamic acid) using Masshunter but I am still seeing high variation in MSDial. But when I did just regular MS1 without any deconvolution, I am getting really good %CV's.
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
Also, we usually do not acquire anything from 0-0.2. It just goes to waste. And so, just to be clear, currently, there is no remedy yet for this. I am glad we found the culprit but too bad there is no remedy yet. What is the process for requesting this for MSDial? Thanks and keep safe.
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
Thank you for letting me know that you got my 3 technical replicate data on 2 ppm negative mode HILIC assay I have attached some of the data processed from Masshunter (molecules' area, height, RT and their corresponding %CV's, which are very low). Scaffold and XCMS have similar low %CV's while MS Dial has %CVs >30%. I have doubled checked the parameters and tweaked them but %CV's were still high. I hope you can help figure out what's going. My parameters used are on previous attachments. Thank you and keep safe.
Regards,
Marites
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
I did what you have asked me and I am seeing the same irreproducibility. Please check my attachment. Maybe I should check the converter. Thanks!
Marites
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
Please see attached picture of the EIC of phenylalanine from masshunter.
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
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MS-DIAL / Re: MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
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MS-DIAL / MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
Sample info:
Calibration curve in methanol: 0, 0.05, 0.1, 0.5, 1, and 2 ppm with 12 injections each levels and ran this in All ions pos and neg in Agilent 6545 HILIC chromatography.
Problem: I am seeing high %CV's from MS-Dial analyzed data than in Masshunter Qual. I was hoping MS-Dial would give the same if not better quality than Masshunter Qual. Can some one please check my parameters?
For example, at 2 ppm for Arginine neg mode, we are seeing 8.4% CV while in MS-Dial we were seeing 90% CV!!! The retention times were comparable between the two data analysis tools. What is causing MS-Dial to have such high %CV? Your help would very helpful. Arginine and Methionine calibration curves are shown in the attached files. Thank you and looking forward to your response. Keep safe!
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MS-DIAL / Re: Why is not accuracy when MS-DIAL process from raw data to alignment results
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