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MS-DIAL / MS-Dial is giving very noisy data in comparison to Masshunter Qual (Help!)
Sample info:
Calibration curve in methanol: 0, 0.05, 0.1, 0.5, 1, and 2 ppm with 12 injections each levels and ran this in All ions pos and neg in Agilent 6545 HILIC chromatography.
Problem: I am seeing high %CV's from MS-Dial analyzed data than in Masshunter Qual. I was hoping MS-Dial would give the same if not better quality than Masshunter Qual. Can some one please check my parameters?
For example, at 2 ppm for Arginine neg mode, we are seeing 8.4% CV while in MS-Dial we were seeing 90% CV!!! The retention times were comparable between the two data analysis tools. What is causing MS-Dial to have such high %CV? Your help would very helpful. Arginine and Methionine calibration curves are shown in the attached files. Thank you and looking forward to your response. Keep safe!