Hello everyone, Hello Hiroshi,
is it possible to normalize by "ref matched" directly in the software? like TIC of ref matched metabolites ?;
For lipidomics studies, is it possible to normalize by "lipid class of ref matched" directly in the software ?
Best regards
Sebas
Hi Sebas,
this can be performed by selecting "mTIC" normalization method of MS-DIAL normalization option.
However, I found a bug here now... mTIC option now includes "suggested" metabolite's information as well.
Now I have fixed the issue, and I will upload the new version next week.
Thanks,
Hiroshi
Now, the new version is online.
http://www.metabolomics-forum.com/index.php?topic=1576.0
Thanks,
Hiroshi
Thank You very much Hiroshi
Hi Hiroshi, i try the new version of MsDial.
Thank you very much for your rapidity.
When I removed manually some "Ref Matched" metabolites in the show ion table of the alignement result, some "Ref Matched" is removed of the table. But if i apply a normalization (mTIC), save it, export the normalized data, the sum of total of Ref Matched in the Excel is not 1, certainly due to the manually 'Ref Matched" removed.
Have a good day
Séb
Hi,
simply, I wrote the source code to let you know how the mTIC value is calculated.
Here, LibraryID should be more than 0 if a peak is annotated by MSP library.
The postidentificationlibraryID should be more than 0 if a peak is annotated by user-defined text library containing the pair of m/z and retention time. The annotated metabolite having the tag of "w/o MS2" is excluded to calculate the mTIC value.
Thanks,
Hiroshi
for (int j = 0; j < alignedSpots.Count; j++) {
if ((alignedSpots[j].LibraryID >= 0 && !alignedSpots[j].MetaboliteName.Contains("w/o")) || alignedSpots[j].PostIdentificationLibraryID >= 0)
tic += alignedSpots[j].AlignedPeakPropertyBeanCollection[i].Variable;
}
}
Thanks Hiroshi!!
Great job!