I would like to set mzdiff to -0.005. However, the filed seems to only accept 5 characters, so if I want the value to be negative, the smallest number I can put in is -0.01.
Is there a way to increase the filed so that it fits -0.005?
I tried processing two datasets previously used with different parameters (and the extraction worked that first time). However, this second time around, there is an error saying "XCMS Processing Failed". The only thing I changed in the parameter settings is no retention time.
Thanks for checking into this. I am sorry I deleted the dataset before you could reproduce the error. I noticed some files didn't upload correctly, for whatever reason, so I re-uploaded one of the datasets.
However, I am still having problems with the graph of TICs (original), and you can see this under job ID 1952.
Thanks very much for the time you take to answer my questions!
I am trying to rerun some analysis with different parameters. I have uploaded these datasets before and they went through the file check just fine. This time I am getting an error and the process is stuck at the "file check" point. Job ID is 1898.
Also, is it possible to get more space on the web-based XCMS page?
1. There is a problem I don't know how to fix, and this has happened to me a few times now. I ran some data on XCMS online, and Total Ion Chromatograms (original) does not show any peaks, while the corrected graph looks fine.
The ID of the job I ran is 1799.
Can you please help me fix this?
2. Additionally, I am still unable to change any parameters on UPLC/MS without having an error message pop up saying that min peak width has to be less than the max peak width, and so I cannot save the changes.
I am trying to run my data without a retention time correction so that I can compare data before and after the correction. However, whenever I run the data, an error message appears half way into the run.
1) Am I supposed to be setting up some specific parameters not related to the retention time parameters, i.e. feature detection "method" or "integration method" under feature detection? Are these parameters supposed to be something specific for LC/MS data?
2) Also, I would like to be able to change min peak width and max peak width to 5 and 12, specifically, as I am trying to reproduce some data and follow someone else's foot steps for this particular analysis.
Thank you very much! I appreciate your responses! I am new to XCMS but I am very excited about it, and looking forward to using it more and more!
