Areas of EquiSplash Lipidomix February 23, 2021, 01:32:31 AM Hi everyone.I work with green coffee beans, using 15 uL of EquiSplash and 7.5 mg of milled coffee beans.In the lipidomics workflow I have had difficulties to find the deuterated standards, even though I know that they are there in my raw data. Has everyone had the same problem ? Do I need to do something to identified these compounds ? Quote Selected Last Edit: February 23, 2021, 01:50:23 AM by AnaCarolina-RosadaSilva
Re: Areas of EquiSplash Lipidomix Reply #1 – May 24, 2021, 02:51:36 AM Hello, may I consult you a question?I used SPLASH LIPIDOMIX in milk. How can I quantify the lipids?Could I choose splash lipidomix to normalize the data in the normalization of ms-dial? Quote Selected
Re: Areas of EquiSplash Lipidomix Reply #2 – June 07, 2021, 09:15:31 AM Hi. Sorry for my late answer. Regarding quantification, I would rather normalize areas in excel, because MS-DIAL quantification is better to plasma or tissues samples. If you need more help please send me an email: acarolinarosa.16@gmail.com Best Regards,Ana Carolina Quote Selected
Re: Areas of EquiSplash Lipidomix Reply #3 – June 27, 2021, 03:42:41 AM Hi, You can annotate the splash lipidomics standards by e.g. attached format files. (please fix the retention time information.)The attached file is for equisplash mixture.Then, using the SPLASH normalization in ms-dial becomes straightforward.Hiroshi Quote Selected