Hi,
The centWave paper says the following:
But I see others use ppm=2.5, instead of 25, for Orbitrap with advertised mass accuracy < 5 ppm. Could anyone explain why?
Thanks!
I would say that 2.5 is almost certainly a bad idea... It needs to be true also for the edges of peaks to work well.
I used 20 for orbitrap data and I remember that setting it significantly lower gave problems.
Thank you so much for the reply! And thank you for all the tutorials on your page! It's very helpful for a newcomer to this field.
Here is a slide from a tutorial by Workflow4Metabolomics. Seems for Q-TOF instruments, the ppm is set to be ~10 times of actually accuracy, but not for Orbitrap. I also see others using ppm=2.5 for Orbitrap, so I was just wondering why?
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