For those of you who could use a well characterized human plasma sample, NIST has released SRM 1950 for purchase. Please see the product information at:
For those of you who could use a well characterized human plasma sample, NIST has released SRM 1950 for purchase. Please see the product information at:
I posted a series of questions about Standard Reference Materials for metabolomics in the Environmental Interest Group section. Perhaps you could have a look and consider posting your views ...
I posted a series of questions about Standard Reference Materials for metabolomics in the Environmental Interest Group section. Perhaps you could have a look and consider posting your views ...
I would like to query the community about the need for and utility of metabolomics standard materials.
NIST SRM 1950 will be hitting the street soon, but this is a single sample which may be of the most utility to the mass spectrometry community.
For true multivariate metabolomics analysis, one may need a \'set\' of samples that can be analyzed and compared to some reference values. For example, for the NMR intercomparison, we produced two sets of samples: a \'synthetic set\' with six distinct mixtures of six compounds, and a \'biological set\' with a biological tissue extract from an experiment with two discernable treatment groups. These were geared for NMR analysis for this exercise.
So questions: 1) Is there a need for a set of standardized samples produced by an independent agency? 2) What characteristics should this sample set have? 2a) For NMR? 2b) For LC/MS? 2c) For GC/MS? 2d) For other modalities? (Please identify) 3) How would you use such a set of samples? 4) What information would you need about the samples (metadata, chemical info, biological info, etc.)? 5) Have you developed a set of test samples? If so, how did you do that and how do you use it? 6) Should the sample be complex (biological materials) or simple (limited number of components or classes of components)?
Feedback would be crucial to agencies like NIST proceeding with plans along these lines.
Wow! It is pretty tough to think of a future exercise in the midst of a current one... ;-)
BUT! The strength of repeating intercomparisons is that the community learns and grows in confidence and skill through these things. Since the data is reported anonymously, there is freedom to \'give it a try\' and see how your data aligns with the community. If you do well, then this is a great point to include in the next proposal you write or for internal QC needs. If you are not happy with your results, then there is a way to gauge any efforts you make to bring improvement to your process.
Feedback from the community, and citations of the manuscript we will be producing, are the kind of encouragement that we need to forge ahead with planning for another exercise.
I encourage those with ANY interest in participating in the future to contact Mark or me and express that interest, along with any questions or suggestions that you may have.
A second intercomparison exercise has been developed, deployed and executed.
Twelve Laboratories from around the world analyzed NIST-prepared materials using Nuclear Magnetic Resonance (NMR) Spectroscopy.
In all, 16 data sets at four different NMR magnet strengths were collected using standardized protocols.
The experimental protocol involved sample temperature calibration, 1-dimensional and 2-dimensional experiments, multivariate analysis and quantitative evaluation of metabolite concentrations.
Analysis of the results of the exercise is ongoing and should be completed by the fall of 2011.