How Waters's DDA links MS1 peaks to MS/MS spectra July 27, 2022, 09:14:29 PM Dear all,For non-targeted analysis, I perform data acquisition by DDA on Waters's Synapt XS.After converting the obtained data to ABF or MZML, I perform analysis by MS-DIAL.Given the principle of DDA, I would think that the MS/MS spectrum should correspond 1:1 to the MS1 peak.However, I have confirmed several cases where the exact same MS/MS spectrum is tied to multiple MS1 peaks on MS-DIAL.I would like to investigate the cause of the problem, whether the Waters's DDA data is special or the MS-DIAL analysis method does not match the Waters's DDA data.If you have any knowledge, please let us know.I think the key is how Waters's DDA links MS1 peaks to MS/MS spectra...Best regards,Akihiro Quote Selected