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Topic: Getting Started (Read 4191 times) previous topic - next topic

Getting Started

I am just starting to develop a lipidomics method in our laboratory.  I processed control and treated murine brain tissue and analyzed the samples in accordance with the Blankman and Saghatelian Global Metabolite Profiling methods.  I uplopaded the data to XCMS Online and processed them - and that went well - but I am having trouble with the results.  Most of the returned results are false positives in that when you examine the EIC, XCMS has integrated noise.  So my questions are, how can you setup the processing parameters to discard results based on the chromatography of the EIC.  Also, how do you sort or edit the results to essentially accomplish the same thing.