Dear all,
at the moment I have small sets of samples (around 100 per experiment).
The problem I am facing with all my different experiments is after I close the Table viewer for the aligned chromatograms, the program crashes about 20% of the time. I have the feeling, the longer I am in the table viewer, the higher the chances are.
Is there a logfile or a debugging mode I can view/switch on?
Thank you,
all the best,
Martin
Language: English (UK)
Windows 10 Pro
MS-DIAL ver. 4.60
#Data type
Data type Centroid
Ion mode Positive
Accuracy type IsNominal
#Data collection parameters
Retention time begin 0
Retention time end 45
Mass range begin 50
Mass range end 600
#Data processing
Number of threads 4
#Peak detection parameters
Smoothing method LinearWeightedMovingAverage
Smoothing level 3
Average peak width 20
Minimum peak height 1000
Mass slice width 0.5
Mass accuracy 0.5
#MS1Dec parameters
Sigma window value 0.5
Amplitude cut off 10
#identification
MSP file F:\Biology\Libraries\GCMS DB_FiehnBinbase-KovatRI-VS2.msp
RI index file see last
Retention type RI
RI compound Alkanes
Retention time tolerance 0.5
Retention index tolerance 25
EI similarity library tolerance 70
Identification score cut off 70
Use retention information for scoring True
Use retention information for filtering False
Use quant masses defined in MSP format file True
#Alignment parameters setting
Reference file F:\Biology\Data\2021_Macro_ExpII_IntSt-MB\BT TOF\mzml\abf\Pool_1_b1_SL_start_275sec.abf
Retention time RI
Retention index tolerance 20
Retention time tolerance 0.075
EI similarity tolerance 70
Retention time factor 0.5
EI similarity factor 0.5
Identification after alignment False
Gap filling by compulsion True
Basepeak mz selected as the representative quant mass False
#Filtering setting
Peak count filter 0
N% detected in at least one group 0
Remove feature based on peak height fold-change True
Sample max / blank average 5
Sample average / blank average 5
Keep identified and annotated metabolites True
Keep removable features and assign the tag for checking True
#RI dictionary (analysis file path / ri dictionary file path)
cropped
Dear Martin,
In my experience, if I am editing the alignment table (see picture below), and at the same time press Ctrl + D to change the feature to unknown, the program will crash. It happens all the time. So, to change the feature to Unknown in the alignment table, we have to make sure that we are not editing the table.
I don't know if this is your situation. Hope it is helpful for you.
Best,
Sukis
Dear Skuis,
thank you for your reply!
Maybe the problems are somehow connected that you have it while editing the alignment table, and I have it in the table viewer.
Does the crash also mostly/always happen when you close the window?
All the best,
Martin
It does not happen when I close the windows.
Best,
Sukis
Thank you for checking!
All the best,
Martin