Converting LCMS profile data to centroid? January 07, 2014, 03:03:45 PM Hi All-Does anyone know how to do this? We have a stack of about 300 high-res .raw files from our Thermo Q Exactive which we have been processing with SIEVE, but we want to see how much faster it will run using centroided rather than profile data. Quote Selected
Re: Converting LCMS profile data to centroid? Reply #1 – January 08, 2014, 12:51:17 AM Hi,you need to convert to *.mzML anyway, so I'd suggest to do the centroiding as part of the pwiz conversion, although I don't really have hands-on experience there.Yours,Steffen Quote Selected
Re: Converting LCMS profile data to centroid? Reply #2 – January 08, 2014, 04:33:00 PM Thanks Steffen. Why would we have to convert to mzML? The files need to be in the .raw format for the final processing. Quote Selected
Re: Converting LCMS profile data to centroid? Reply #3 – October 01, 2016, 11:04:25 AM Hello I have the same problem.Right click Display Options, over the mass spectra, go to Labels, and select centroid.Sadly the centroid option doesnt show up for me so I cant test this (even though I have profile data).Then you can probably right click, export, write to RAW file.Let me know if that works. Jeremy Quote Selected