post-acquisition centroiding of Waters data September 06, 2017, 03:29:06 AM Hi,I was wondering if anyone knows a way to get good centroiding of Waters data that was recorded in profile mode?In MassLynx you can centroid a single spectrum and it looks like this as an example:That seems reasonable by eye.I tried then using the centroiding in msconvert (Proteowizard). Here is the result:What it has done is not finding the center of the peak but has chosen the top scan for the m/z. This is not very accurate and is in this case a difference of 15 ppm.So my question is if someone knows a better way to centroid data that was already acquired?Msconvert can use vendor algorithms for centroiding for a lot of formats but apparently it is not available for waters data.UPDATE: msconvert now supports vendor (AKA Waters) centroiding.[attachment deleted by admin] Quote Selected Last Edit: December 05, 2018, 03:48:38 PM by Jan Stanstrup