Dear Community,
I am working on a cell-based metabolomic project. Does anyone know how to normalize the data using protein content in MS-DIAL?
Thank you!
Pan
Hello Pan,
do you have additionally an internal standard in your samples?
If yes: IS normalization in MS Dial, then take the alignment sheet and divide your samples by the corresponding protein amount.
If not: take the alignment sheet and divide your samples by the corresponding protein amount
You can do a TIC / mTIC normalization, but I'm not very confinced it has a lot of benifits.
All the best,
Martin
Thanks Martin!
Yes, I have an internal standard, will do as you suggested.
Best,
Pan