Dear Sir,
I am a PhD student at Heidelberg, and currently I am working on nLC-Exactive to quantify the sphingosine bases.
My questions is while its very simple to create a MRM method with the Q-trap instruments, I dont know how establish this method with Q-E,
Mass transition
C17Sph 286/268 7.2
Sph 300/282 7.5
DHSph 302/284 7.6
S1P 380/264 8.7
DHS1P 382/284 9.0
C17S1P 366/250 8.2
Should I choose Targeted - SIM or data dependent?
I would appreciate if someone could share his/her experiences.
with my best regards.
Cagakan