pSRM with Q-exactive February 08, 2014, 06:50:12 AM Dear Sir, I am a PhD student at Heidelberg, and currently I am working on nLC-Exactive to quantify the sphingosine bases. My questions is while its very simple to create a MRM method with the Q-trap instruments, I dont know how establish this method with Q-E, Mass transitionC17Sph 286/268 7.2Sph 300/282 7.5DHSph 302/284 7.6S1P 380/264 8.7DHS1P 382/284 9.0C17S1P 366/250 8.2Should I choose Targeted - SIM or data dependent? I would appreciate if someone could share his/her experiences. with my best regards.Cagakan Quote Selected