I have analyzed Drosophila melanogester polar extract using GC-MS after methoxyamine hydrochloride derivatization and TMS protection. Due to high abundance of disaccharide peak intensities, i am unable to solve their mass spectra with the help of NIST and willy libraries. I have also analysed 4 standards of disaccharides. But the sample peak Rt were not matching with the standard Rt. I am using Thermo Quantum XLS triple quadrapole mass spectrometer. Can anay one suggest to identify disaccharides.
