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MS-DIAL / Internal Standard Question and "Find" option in MS DIAL v4.16
I am having some difficulty understanding what input values to place when attempting to normalize data. Using a SPLASH mixture of known concentration, when I select to normalize the data there are default values in the "SPLASH mixture settings" window. Should I change the values according to how much was spiked into the sample? When inputting the values, do I need to take into account injection volume?
Also, when attempting to use the "Find" button, several, if any of the standards do not appear in the list or are labeled "w/o MS2". However, if I were to manually look for them in data acquisition software I am able to locate the precursor and MS2 spectra of the standards of interest.
Thanks.