MS-DIAL / Re: Identical lipids with same retention time in Bruker PASEF data (MS Dial 4 paper)
It works now! Thanks. Will update if I have more questions.
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Dear users,Hi Hiroshi,
Sorry for my late action. Yes, Shuai is right.
As the procedure of MS-DIAL, the program detects the peak in the RT axis. Then, the ions are expanded in the drift axis. Then, the peaks are detected in the drift axis. The row containing -1 value means that this is the result of RT axis peak detection. Therefore, you will see the same RT results twice.
Now, for bruker tims-tof data, you do not have to convert .d file into ibf. (I hope)
From next version (it will be uploaded in the mid of July), the MS-DIAL also supports the direct import of .d file containing pasef data.
You can evaluate it by the following link. (the link is expired on 4 July, 2021)
Hello, I am working with ion mobility data (from agilent LC-IM-QTOF) and this happened as well.Hi Ana, thanks for the reply. Just checked the MS Dial tutorial again and it seems that the same lipid is reported twice, once in RT vs m/z dimentions and once in ion mobility vs m/z dimentions. So far, it doesn't like there is any easy way to filter out one of these two groups within MS Dial data viewer.
I only excluded all the -1 compounds, it seems for me that it is a bug from the system and the lipids are duplicated.
Hope it help you.
Ana Carolina Rosa
How did you convert and import the data?I used the ibf converter which comes with MS Dial 4.60 and selected ion mobility as data type.