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Messages - Sara

1
MS-DIAL / Re: RIKEN lipids identified
In the case this kind of lipids are regulated in our results how can we assign them an ID?
Or do you suggest we can delete the "RIKEN ID" from our final results?

Thanks in advance
2
MS-DIAL / Re: RIKEN lipids identified
Thank you for your reply. Now, I've understood.
About the BinVestigate button in MSDIAL I can't use it because it works only for GC-MS projects.

Best regards


3
MS-DIAL / RIKEN lipids identified
Hi All,

I am attempting to analyze a lipidomics DDA dataset that was acquired on an orbitrap QE plus instrument. I converted the *.raw file to *.ABF files. I've noticed that in the final alignment some of the compounds were annotated as such: "RIKEN P-VS1 ID-7082 from Mouse_Macrophage_WT_N_F1AA", but in the identification step (i'm working in lipidomics mode) these kind of lipid are not presents. Is there a database somewhere where I can search for the molecules associated with this type of annotation? How do we can use this information of ID's?

Thanks,
Sara
5
MS-DIAL / Re: Problem with post-id
"only report the top hit" is checked.
I mean that i can't find the two m/z in the alignment list of the "Alignment spot viewer".
Thank you very much

Sara
6
MS-DIAL / Re: Problem with post-id
Hi,
It's not a problem linked with the language, because the identification with LipidBlast correctly run and i have a great number of Ref. Matched.
it is in the post identification settings maybe, because only 8 out of 10 of the lipids in the list are shown in the final alignment.
7
MS-DIAL / Problem with post-id
Hello,
I'm using MSDIAL 4.20 and in the final alignement of my data i can't find all of my lipids listed in the txt. file for the post identification.
I'm am sure that all of them are present in all of mine samples because I checked manually the XIC.

I'm using an identification score cut off of 80%
Any suggestion?