Thank you so much for your reply. Actually I splitted the samples into small groups (say, less than 100 files per MS-DIAL analysis) and managed to run them. Now the issue is how to combine these aligned data together...
I tried to analyze more than 300 mzML files and encountered the same error (Error in JointAligner, alignment file not found). Actually, (.pai2) and (.dcl) files were created for each mzML file, but no (.aef) and (.arf2) files were created. I think this is a memory issue and thus, I need to lower the number of mzML files per analysis (maybe 50 per analysis) and join the alignmentResult files later on Excel or something. My question is if there is any way to resume the analysis from (.pai2) and (.dcl) files on MS-DIAL to save time. Or do I have to start from scratch (I mean, uploading mzML files)?
Thank you very much in advance for your kind help and suggestion!
Hi oscardr- Thanks for your response. I am from the US and the OS is the English version. Now I am using a different PC with the same OS (Windows 10 Pro Version: 20H2) but a faster CPU (1.80GHz vs 2.83GHz) to get around this issue. Is CPU (@1.80GHz (.99 GHz) too low for MS-DIAL? KenT
I am a newbie of metabolomics, informatics, and MS-DIAL. MS-DIAL keeps crashing soon after I set up a new project in the metabolomics mode and hit "Finish". The situation is similar to this previous thread. http://www.metabolomics-forum.com/index.php?topic=1628.0
I tried both versions (4.60 and 4.48) in vain. But both versions ran well without selecting any msp file in Identification tab. After the run, I repeated the analysis from the Identification step with choosing a correct MSP file (MSMS-Public-Pos-VS15.msp), but it crashed within seconds. Obviously, it keeps crashing during loading a MSP file.
Here is the detailed info about my PC and files (I am not sure whether these are relevant or you need other pieces of info). Edition: Windows 10 Pro (English) Version: 20H2 RAM 16 GB mzML files after conversion from Waters .RAW files. Virtual memory: Automatically managed paging file size for all drives. Currently allocated 12898 MB
Any suggestions are highly appreciated! Thank you very much in advance.
