Hi to all I have the following doubts about the analysis of All Ion Fragmentation data (AIF) using only 1 energy collision: I have the following doubts about All Ion Fragmentation (AIF) data analysis: 1) My ms2 data are obtained with only ONE energy collision. I need to use the "SWATH-MS or conventional All-ions method" or I can use the "All-ions with multiple CEs method". If I use the second method, is it correct to use the "Experimental_Information_ForAIF.txt" shown in example 2 (the picture is from the MS-DIAL tutorial)? 2) How can I understand the order and the value in the “experiment” column, since in the tutorial "Tutorial 2. Compound identification in LC-MS AIF data using AMRT MS2 library 191018" is written: "the experiment ID is listed (by order of acquisition, usually vendor-specific)"?
Hi to all I want to acquire samples in full scan mode and QC in full scan and "full scan + AIF mode" (with a single CE). Is it possible to analyze the full scan data and "full scan + AIF mode" in the same MS-DIAL project? Thanks Ruggiero
Hi to all. I am trying to use MsdialConsoleApp to improve the robustness and speed of gcms data analysis. I am currently unable to parse the gcms file in the tutorial (http://prime.psc.riken.jp/compms/msdial/consoleapp.html), as when I run the software it stops printing the "The given key '0' was not present in the diction" as you see below.
MsdialConsoleApp.exe gcms -i .\GCMS\ -o .\GCMS\ -m .\GCMS\Msdial-GCMS-Param.txt -p Loading library files.. Start processing.. Reading data... Exporting Deconvolution data: .\GCMS\140428actsa25_1.msdial .\GCMS\140428actsa25_1.cdf finished Reading data... Exporting Deconvolution data: .\GCMS\140428actsa26_1.msdial .\GCMS\140428actsa26_1.cdf finished Reading data... Exporting Deconvolution data: .\GCMS\140428actsa27_1.msdial .\GCMS\140428actsa27_1.cdf finished The given key '0' was not present in the dictionary.
Everything works fine if I leave only one sample in the GCMS folder and analyze it, as you see below.
MsdialConsoleApp.exe gcms -i .\GCMS\ -o .\GCMS\ -m .\GCMS\Msdial-GCMS-Param.txt -p Loading library files.. Start processing.. Reading data... Exporting Deconvolution data: .\GCMS\140428actsa25_1.msdial .\GCMS\140428actsa25_1.cdf finished Now saving the project to be used in MSDIAL GUI...
I'm working on Windows 10 Home with CPU Intel(R) Core(TM) i7-1065G7 CPU @ 1.30GHz 1.50 GHz and RAM 8.00 GB (7.81 GB usable), but I have the same error on Linux and macOS. Do you have any idea?
