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Sample preparation / Protecting -SH groups
In our experiments we often see -SH compounds (such as reduced glutathione - GSH) disappear and form disulfide bonds (GSSG in the case of glutathione).
To prepare our samples we use 50% methanol extraction, followed by 10-kDa cut-off filter to remove proteins, followed by vacuum evaporation.
I am wondering if other researchers experience this problem and how they deal with it. Do you add any buffers to your extraction medium?
Regards,
Tomas