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Topics - DeborahMaus

1
Other / MAVEN
Dear Metabolomicians!

Does anyone of you use MAVEN?

I am having problems visualizing data. I ran an untargeted experiment with data containing MS1 and MS2 (Top10) spectra (QE Thermo Fisher). I converted the .raw files to .mzXML and opened it in MAVEN.

In the attached screenshot you see the dashed TIC (why can't I get it smaller? How can I change the appearance?) and the EIC for the positively charged glutamate ion. Believe me, in Compound Discoverer or the Qualbrowser glutamate gives a strong and beautiful peak in the same dataset.

Do you have a hint what I should be looking for? Or does MAVEN simply not work with untargeted data from MS1+2 Top10 experiments?

Best,
Debbie.
2
Good morning fellow Metabolomicians!

We observe a Retention Time drift but a minor one. And now, while I am in the middle of optimizing, I am thinking:

Would it be better to change the apolar eluent from pure AcN to AcN with 5% water containing 10mM Ammonium Carbonate?

Thanks in advance for your effort to help to newbee! :)

Greetings from Germany!
Debbie

P.S.: HILIC mode! Polar eluent: 10mM Ammonium Carbonate in water.