I am trying to analyze DIA-Pasef data (plasma lipidomics, RP-UHPLC) with MS DIAL v4.80 I have followed the usual workflow suggested for IMS-DIA, but I have basically 0 hits. Do I am missing something? The same samples were analyzed with DDA-Pasef, and everything worked. Thank you in advance for your help Best Eduardo
HELLO ALL I HAVE A QUESTION FOR MANUAL CURATION OF MSMS DATA BETWEEN DOT PRODUCT, REVERSE DOT PRODUCT AND TOTAL SPECTRAL SIMILARITY, WHICH PARAMETER "WEIGHTS" MOST? APART FROM EVIDENT SPECTRAL SIMILARITY/DIFFERENCE, WHEN DO YOU CONSIDER A SCORE "GOOD" THANK YOU IN ADVANCE
