Hi everyone. I work with green coffee beans, using 15 uL of EquiSplash and 7.5 mg of milled coffee beans. In the lipidomics workflow I have had difficulties to find the deuterated standards, even though I know that they are there in my raw data. Has everyone had the same problem ? Do I need to do something to identified these compounds ?
Hello. I am trying to process LC-IM-QTOF data in the MS-DIAL 4.48, but the time to end has been higher than I was expecting. I am processing only two lipid samples, because it is only a test. This is my first time with ion mobility, but I have been using MS-DIAL for my first work using lipidomics and orbitrap.
The computer is a desktop with 192 GB of RAM.
Do you have long processing times for ion mobility data ? More than 3-4 hours ?