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Topics - ThmsBrnt

1
MS-DIAL / MS-DIAL v5.1.230807: LOWESS unavailable when trying to normalise
Hi,

I am currently experiencing an issue with MS-DIAL v5.1.230807. After processing my SWATH data, including peak detection, deconvolution, identification, and alignment, I have encountered a problem with the normalization step using LOWESS. The "Normalize" button appears to be greyed out and inaccessible.

I want to note that I have QCs available and that I have configured the settings for the overall data, including analytical order and sample type. Despite this, the "Normalize" button remains inactive.

Has anyone else encountered a similar issue and found a solution? Your insights would be greatly appreciated.

Thank you.

Thomas
2
MS-DIAL / MS-DIAL V5.1.23 : crashes after adding a .msp/.txt file for identification
Hi,

I have a SWATH-MS/MS metabolomics dataset processed with v4.9 and I want to compare the results with v5.1.23. When I try to process the data with MS-DIAL 5 the software crashes before doing anything after clicking "Run". I figured out that it was because of the "Identification" module. If I don't use it, the software works properly: I effectively end up with aligned spot and etc. But I can't seem to be able to identify my MS/MS spectra with a .msp/.txt database as it always crashes the software even when doing it after processing (peak peaking, adduct identification, deconvolution, alignement, etc.).
Does anyone else have this issue and could enlighten me?
Thanks.

Thomas B.