Hi sorry I am late to the game here but what type of analysis do you want to do. You can do precursor ion scan (PIS) or neutral loss scan (NL) experiments to capture lipids of interest of a particular class. Seems you want to be more discovery than targeted and the QT5500 is a great platform for this type of workflow. There is on the sciex.com website an application note you can download or alternatively if you email me I can send it to you which allows a multiplexed precursor ion scan (MPIS) method allowing for comprehensive coverage of the lipidome. Thanks Bal