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Messages - mihaicurtasu

1
METLIN / Re: METLIN MS/MS spectra access?
Hi !

Actually, I never received any answers, and starting this year I moved to the Gen2 spectral library because I really needed the library access.

All the best!
2
METLIN / METLIN MS/MS spectra access?
Hello everyone,

Is anyone else experiencing issues to open the MSMS spectra from metlin (metlin.scripps.edu). Search function works great, just as before, but not opening the MS/MS spectra when available. Also other memebers from my lab cannot open the spectra. I've seen now they're moving also on a commercial site, but now I am talking about the old version of metlin.
I've sent an email to contact asking about this issue, no replies yet...

Cheers!
3
XCMS Online / Re: CAMERA annotation
Indeed I was refering to the adduct/fragment annotation. I will contact them directly.

I am in the process of setting up the offline R XCMS, I started by using the online version for convenience :).

Thank you very much Jan!
4
XCMS Online / CAMERA annotation
Hi all,

I hope i can get a solution to this issue i'm having in XCMS online.

I've been submitting jobs for the last days and for all of the jobs i have received the following note:
"2017-02-14 05:55:48 : Too many features. Processing data without annotation."

Why does this keep happening? I've been analysing urine, serum, liver tissue samples, and frankly, looking at the number of features
they don't look so far off normal. Usually for urine I get around 4000-5000, serum and liver I have around 2000.

In the final table it generates, some of the features are marked as [M+1], [M], sometimes [M+2] and the coresponding in negative mode. This can't be the proper annotation, or full (i might say), because i'm sure there are features there that must be [M+Na], [M+K]... etc.

Any ideas how I could get the annotation to properly work?

Thank you !