Hi, I have basically the same issue, I recently posted it After file conversion and alignment, that works fine, I cannot extract MS/MS features, so basically I get 0 hits since MS/MS is totally absent. @Hiroshi Tsugawa could you help us? Kind regards
I am trying to analyze DIA-Pasef data (plasma lipidomics, RP-UHPLC) with MS DIAL v4.80 I have followed the usual workflow suggested for IMS-DIA, but I have basically 0 hits. Do I am missing something? The same samples were analyzed with DDA-Pasef, and everything worked. Thank you in advance for your help Best Eduardo
I am having the same problem reported by Stefano, in LC-q-exactive platform (HILIC chromatography) Basically I have my compound split over adjacent scans, and thus multiple identification features for a same compound, clearly this is related to the peak width, that moreover, usually in HILIC is wider than RP How did you solve this situation? Kind regards Eduardo
HELLO ALL I HAVE A QUESTION FOR MANUAL CURATION OF MSMS DATA BETWEEN DOT PRODUCT, REVERSE DOT PRODUCT AND TOTAL SPECTRAL SIMILARITY, WHICH PARAMETER "WEIGHTS" MOST? APART FROM EVIDENT SPECTRAL SIMILARITY/DIFFERENCE, WHEN DO YOU CONSIDER A SCORE "GOOD" THANK YOU IN ADVANCE
