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Messages - Andrew Morris

1
MS-DIAL / Adduct identification in MS-DIAL
I have been using MS Dial to process lipidomics data collected using a reverse phase UHPLC/TOF MS workflow.  I have data sets collected using survey TOF MS and data dependent MS/MS and TOF MS with SWATH MS/MS methods.  The data quality is good and I can observe several abundant lipid species can be observed I process the data manually using Sciex OS.  I am still trying to optimize lipid identification using MS-DIAL, particularly for the SWATH data sets.  One issue that is very vexing to me is that MS-DIAL does not identify ammoniated adducts of mono di and tri glycerides even though these are present in the data and checked in the processing set up (and I have selected the ammonium formate solvent option).  Is there something trivial I am missing here?  Are there other "tweaks" for processing this type of TOF MS data that would improve my lipid identification?  Thanks in advance for any help.