1
MS-DIAL / ms2Dec suggestion
I am using
Software name and its version: msdial 4.24
MS raw data file (a representative one): 1 ms1 followed by 7 swath experiments (window=50Da), collected by Sciex X500R (qtof). Raw file was converted with msconvert3.01 to mzML (peak picking 1-, 32bit, zlib).
I am trying to identified metabolite features in swath data using inhouse curated ms2 library.
While FAQ says that msdial doesn't support sciex swath data, it works for my file as long as i set the peak detection threshold to 10 counts otherwise ms2dec won't work (generate triangle chromatogram). It is fine for us but just costs a lot computing resource. Is it possible to separate ms2 peak detection from ms1, since the intensity of most of ms2 scan is lower than of ms1?
Thank you a lot, this is a great software,