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Messages - ethidium

1
MS-DIAL / peak splitting: I want less peaks.
Hello,

I'd like to ask for help. I hope there is an easy answer.
My lipidomics abf files are quite big by themself (1+ Gb from QToF MSe), and the alignment is also very rich (13K features). Many of them come from peaks being split or picked as separate entities. In example attached below, there are four peaks. In some cases they have equal areas in some samples and in another subset of samples values can be very low or very high. Inspecting raw data on MassLynx show that they are in fact a single analyte.
http://www.metabolomics-forum.com/index.php?action=dlattach;sa=tmpattach;attach=post_tmp_16020_eaa3aec5bf9a284059c7925ca1df2a57;topic=0

Is there a setting a can play with to make sure the alignment will produce one instead of many features? I have tried changing smoothing, but without much success. Min. height = 1000.
Please help,
- Mariusz
3
MS-DIAL / Re: Abf Converter Error
Just a thought: maybe the LockMass (LeuEnk) is doing the problem. Still I havent found  a solution to it.
4
MS-DIAL / Abf Converter Error
Hi,

I wanted to covert my Waters' .raw files to .abf. It works fine for files in positive polarity, but in negative I have got an error message and the batch is interrupted.
 The relevant line of the message says:
<< numPeaks 8013 is different from peaksInScan 8014 >>

Somehow it skips a row and stops. Is there way to do something about it?
Cheers,
Mariusz