I am trying to upload the list of internal standard from the equiSPLASH mix in MSDIAL5 and I'm unsure where to load it in the new software.
1. Do I add it during the Identification Step as a "Text" Database? 2. If yes, what is the suggested template for it? The 4.9 template does not seem to work anymore and the software closes without any error messages after I click "Run."
The problem has been sorted! It turns out the layout of my post-identification .txt for my SPLASH internal standards were not in the correct layout. It just never proceeded forward without a warning.
I recently collected data on an Agilent 6545 QTOF in negative and positive mode. The analysis of the positive mode data ran fine - I was able to get to the main screen after searching and alignment. However, the same data set but in negative mode, I wasn't able to get passed the Analysis parameter setting. I set it up the same exact way (albeit for negative mode).
I have tried versions 4.90 and 4.92, and on different computers and have obtained the same result.
