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Messages - brynn

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Sample preparation / Strategies for resuspending in small volume after dry-down
I am quite new to lipidomic sample prep, and I am wondering what strategies people use to get complete resuspension of their sample after drying down from a large volume.

An MTBE extraction protocol I'm looking at dries down 14 mL of solvent in a glass tube and would be resuspended in ~400 mL. Obviously internal standards can help control for losses, but what other tricks/techniques do you use to ensure the best recovery possible in this situation?

Thanks!