Thank you everyone for your responses. I tried all your suggestions and other advice I found online but none seemed to work. I am still trying to find solution to that particular data while continuing with other tissue samples. And I will let you know when I am able to figure it out.
Thank you very much, Dr. Biswa for your response. I appreciate it.
To be more clear on my previous question. Actually, it's not that MS DIAL did not detect any hits. It did detect over 28,000 hits but after I compared the hits with the reference library, it did not display any peaks with the reference spectra. The hits were reduced to zero. Please see the two screenshots showing hits generated, and zero hits after I compared them to the reference library.
I am trying to run my sample in the lipidomics mode but I got zero matches. It showed no peaks matched with reference libraries. Could you please suggest what could be the problem and how to solve this?