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Sample preparation / Re: Protecting -SH groups
But during the sample preparation, once cells are broken, GSH is exposed to air and oxidized into GSSG very quickly.
Adding reducing agent into sample converts GSSG into GSH, then only GSH will be detected. Therefore, I don’t think there is a good way in sample preparation for these “active” molecules, any metabolomics results on these active molecules should be careful.
if you are only interested in GSH/GSSG, you can use a fluorescent assay to measure GSH/GSSG. You only need minimal sample preparation for assay measurement.