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Messages - Sebas

2
MS-DIAL / Re: MS-DIAL 4.60 was released.
Dear Hiroshi,
Thank you very much for this software and your investment in improving the software.
I downloaded the new version of Ms Dial (4.60).
When I open an MsDial and fill in my working folder and want to continue I get this error message. Have you ever encountered this problem?

Best regards

Sebas
6
MS-DIAL / Re: Normalization
Hi Hiroshi, i try the new version of MsDial.
Thank you very much for your rapidity.

When I removed manually some "Ref Matched" metabolites in the show ion table of the alignement result, some "Ref Matched" is removed of the table. But if i apply a normalization (mTIC), save it, export the normalized data, the sum of total of Ref Matched in the Excel is not 1, certainly due to the manually 'Ref Matched" removed.

Have a good day

Séb
8
MS-DIAL / Normalization
Hello everyone, Hello Hiroshi,

is it possible to normalize by "ref matched" directly in the software? like TIC of ref matched metabolites ?;

For lipidomics studies, is it possible to normalize by "lipid class of ref matched" directly in the software ?


Best regards

Sebas
9
MS-DIAL / GC-MS (QQQ)
Dear Hiroshi, Dear Ms_dial user,

Recently I tried to use MS-Dial to analyze GC-QQQ data (Standard of Fames), acquired by Thermo QQQ.
Here I compare 2 Fames standards with 2 blank.
I set the parameter "Minimum peak height" at 1E06. (see parameters_1.png)
After the alignment I checked all the peaks.
As expected, I note that in the blank, there is no peak C18: 3n6 (Peak Id :-2). (see Picture_2.png).
Although the software automatically takes background noise for Blank, the peak heights are less than 1E06.
Having set the parameter "Minimum peak height" at 1E06, I wonder why the blank value is not 0?

Sebas
12
Sample preparation / Quantification relative vs quantification absolue
Dear all,

Sorry for this basic question, but i need our advice about relative lipids quantifiation between two sample.
I'm beginner.

Imagine that we started with the same starting quantity of a sample (weight (ie 10 mg DW).
We wish to compare samples A versus B.
No internal standard during extraction
Injection using LC-QTOF ddmS2, data procesing using MS_Dial and Lipid blast for annotation.
Is it possible to perform a relative quantification between A and B (normalization to total amount of a lipid class or all measured lipid classes) ? or an internal standard need to be put during extraction process ?

Sorry for this basic question
13
MS-DIAL / Relative quantification of lipids
Dear all,

Sorry for this basic question, but i need our advice about relative lipids quantifiation between two sample.
I'm beginner.

Imagine that we started with the same starting quantity of a sample (weight (ie 10 mg DW). We wish to compare samples A versus B.
No internal standard during extraction
Is it possible to perform a relative quantification between A and B (normalization to total amount of a lipid class or all measured lipid classes) ?

Sorry for this basic question

15
MS-DIAL / Database
Dear community,

I have samples which consist of lipids (TG, PC, PE, CL) which are not present in the database (unusual fatty acids). I would like to create a * txt or * msp file of MSMS spectra of my lipids automatically.
Is it possible to do this using the lipidblast template to generate in silico MSMS spectra of my PC, PE, TG, CL having unusual fatty acids?
Best regards,

Sebas