Metabolomics Society Forum

Software => MS-DIAL => Topic started by: dawal on August 21, 2020, 08:09:37 AM

Title: MS Dial - SWATH no MS2 Data
Post by: dawal on August 21, 2020, 08:09:37 AM
I have a problem with generating an All-ions-project (SWATH) in MS-Dial. I measured with a Bruker QTof and at the moment I use MS-Dial ver. 4.24 but I also tried it with older Versions. MS-Dial is not able to find MS2 features, although when looking at the mzml-files with seeMS clearly MS2 data was measured. Additionally I tried to use old data which I already analyzed with MS-Dial but for some reason it does not work anymore. Here also no MS2 data can be found.
I think my experiement file is correct and do not know what I could change to make it work. Does anyone have an idea what might be wrong?

I have attached my experiment file and also can provide other information if necessary. The experiment file has two Scan-Experiments but this worked in the past.