I was trying to export alignment to an msp and got an error I wasn't sure what to make of (see below). Can anyone tell me what causes this?
Dear Matthew:
Did you do something like this?
1. You processed your data with e.g. MSP file A for peak annotation, and then, created an alignment result A.
2. Then, you reprocessed your data (in the same project) with MSP file B for peak annotation, and then, created an alignment result B.
3. Then, you tried to export the alignment result of "A".
If yes, the outofrange exception is due to the difference of MSPs.
Although the MSP ID stored in the alignment result A is for MSP file A, your current project (.mtd2 file) now contains the information of MSP file B. When the file sizes of MSP file A and B are different, this kind of error will be occurred.
Does it make sense for you?
Hiroshi
Hi Hiroshi,
thanks for the detailed answer. I have exactly the same problem and you described the situation I am in. Now, I am not sure I understood what I could do to export the "alignment result B" (obtained with the MSP file B). Should I just start a new project using the same parameters so the feature list won't change (I hope) but this time it will look for annotations with MSP file B?
Cheers
Jean
Hi Jean:
>>Should I just start a new project using the same parameters so the feature list won't change (I hope) but this time it will look for annotations with MSP file B?
Exactly. At this moment, I recommend, you should start a new project if you wanna use a different MSP file. (it is actually true in lipidomics project. If you wanna change targeted lipid subclasses, better to start a new project)
Thanks,
Hiroshi
Hi,
thanks, will do like that. If possible in future versions that might be useful to improve to allow users to easily look for the same features in different msp libraries (just an idea :) ).
Thanks again for the help
Jean
Good night, this error has appeared when I try to use it, What can it be?
For this, the reasons should be
(1) wrong polarity setting
(2) minimum amplitude cut off (in peak detection tab) should be lowered more.
(3) an destroyed file is imported.
I hope this information helps you.
Thanks,
Hiroshi