Hi Hiroshi,
It happens randomly to me that the MS-DIAL's feature intensity is inconsistent. Sometimes it will select the highest point as the peak height. But sometimes it will show a different intensity (I can't predict either peak height is higher or lower, is there a algorithm behind it?). Please see attached screenshot 1-2 for examples.
However, after manual peak integration, the peak intensity is always the highest point in the selected window (Shown in the Screenshot 3).
I noticed such phenomenon of inconsistent peak height (peak area) in lots of my processed feature table. Is such inconsistency an underlying problem? I am not sure what is going on. Thank you very much for your help!
Best,
Zixuan
maybe this is related to smoothing - different parameters in smoothing will result in different peak heights using same raw data
Thanks for the help! The problem is that after manual integration of the same area, the peak intensity changed. I think it should be a reproducible process (Integrate the same peak should give you the same result).
Just not sure what is happening.