Hi All-
Does anyone know how to do this? We have a stack of about 300 high-res .raw files from our Thermo Q Exactive which we have been processing with SIEVE, but we want to see how much faster it will run using centroided rather than profile data.
Hi,
you need to convert to *.mzML anyway, so I'd suggest to do the centroiding
as part of the pwiz conversion, although I don't really have hands-on experience there.
Yours,
Steffen
Thanks Steffen. Why would we have to convert to mzML? The files need to be in the .raw format for the final processing.
Hello I have the same problem.
Right click Display Options, over the mass spectra, go to Labels, and select centroid.
Sadly the centroid option doesnt show up for me so I cant test this (even though I have profile data).
Then you can probably right click, export, write to RAW file.
Let me know if that works.
Jeremy