Cross MS-Platform Quantitation with XCMS, HOW? July 15, 2011, 11:16:40 AM Hello Again,centWave handles data from a Bruker MicrOTOF-Q instrument and also from Thermo LTQ -Orbitrap data.We are really impressed with its feature detection performance. Is the acquisition of spectra information different for those machines and how does centWave compensate for that?For example, we believe that Thermo uses an instantaneous model of aquisition, where there is biological sample missedbetween the centroids and they account for that by just integrating "a connect the centroids model". See the red area figure I made up.On the other hand there could be a different acquisition mode, right? We believe its also possible to have an "open door" acquisition mode, where each centroid represents the cumulative sum of ions acquired between the last scan and the current scan (blue area in figure). And is centWave's normalization of scan rate to compensate for inter or intra sample scan rate variability? Please see recent post under"area under curve" of a guess at how you might do that normalization on an intra sample case.