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Topic: Metabolite identification with the Q Exactive and LTQ Orbitrap Elite Course (Read 104 times) previous topic - next topic

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Metabolite identification with the Q Exactive and LTQ Orbitrap Elite Course
Venue: Birmingham Metabolomics Training Centre, School of Biosciences, University of Birmingham, Birmingham, UK.
Date: 22-23 November 2018
Level: The course is suitable for PhD students and post-doctoral researchers who have been actively applying metabolomics for a minimum of 6 months. If students or researchers would like to take the course but do not have the recommended level of experience please contact the course administrator for advice.

Overview
This 2-day course provides a hands-on approach to teach attendees about the latest techniques and tools available to perform metabolite identification in non-targeted metabolomics studies. The course is led by experts working within the field of metabolomics, and will include a significant proportion of hands-on experience of using mass spectrometers, software tools and databases. A maximum of four people will be working on each mass spectrometer (Q Exactive and LTQ-Orbitrap Elite) in a session.

Topics include:
  • Importance of mass spectral interpretation
  • Types of data which can be collected on the QE and LTQ-Orbitrap Elite (m/z, retention time, MS/MS, MSn)
  • Conversion of raw data to molecular formula and putative metabolite annotations
  • MS/MS experiments in metabolic phenotyping for on-line data acquisition using the QE (Data Dependent Analysis, Data Independent Analysis)
  • MS/MS and MSn experiments for sample fractions using the LTQ-Orbitrap Elite
  • Mass spectral libraries (using mzCloud)
  • Searching mass spectral libraries
  • Tools for mass spectral interpretation
  • In silico fragmentation (using MassFrontier)
  • Reporting standards for metabolite identification
  • Question and answer session with the experts

For further information and registration details, please visit https://www.birmingham.ac.uk/facilities/metabolomics-training-centre/courses/metabolite-identification.aspx or contact bmtc@contacts.bham.ac.uk.
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