Skip to main content
Topic: MS-DIAL -HDMSE dataprocessing problem (Read 452 times) previous topic - next topic

MS-DIAL -HDMSE dataprocessing problem

Dear all,

I am very excited to analyze my samples, which are acquired in LC-ion mobility-MSE (LC-HDMSE) using MS_DIAL 4.16 version. However, as I start to generate a new project and load ".abf" files, it doesn't show anything on the next page. I am following the steps in online tutorial and not sure what I am doing wrong.  thank you so much for your help.

Sincerely,
Fereshteh

Re: MS-DIAL -HDMSE dataprocessing problem

Reply #1
Hi Fereshteh,

the abf converter does not work for ion mobility data. Instead, please use "IBF converter": you can find it in the same folder of MS-DIAL program. MS-DIAL supports the ibf format file as the input for ion mobility data processing.

Thanks,

Hiroshi

Re: MS-DIAL -HDMSE dataprocessing problem

Reply #2
Dear Hiroshi,

Thank you so much for your prompt response! I converted the ".raw" files to ".ibf" files; however, it still doesn't show anything on the next page of a "new project window".

I will be happy to share more information or my data files if it helps to figure out the problem.

Thanks for your help,

Sincerely,
Fereshteh

Re: MS-DIAL -HDMSE dataprocessing problem

Reply #3
Please see step 1 and step 2 for you.:D

Re: MS-DIAL -HDMSE dataprocessing problem

Reply #4
Thank you so much! Here are the screenshots of the steps that I applied. The data files are acquired in ion mobility profiling mode with ramping collision energy from 25 to 65 eV at the transfer region of TWIMS.

I appreciate your help,

Sincerely,
Fereshteh

Re: MS-DIAL -HDMSE dataprocessing problem

Reply #5
hi,
I have the same problem with you !!! did you find a solution ??
salutations !

Re: MS-DIAL -HDMSE dataprocessing problem

Reply #6
Hi,

for last step (step 3), did you click "browse" button for selecting ibf files?

Hiroshi