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Topic: normalization (Read 3477 times) previous topic - next topic

normalization

Hi Hiroshi,

Question about normalization (performed with LOESS + IS).
In my Alignment results I have a total of 3300 features. When I export the results of normalization (performed with LOESS + IS),  however, in the pdf report I only see 365 features (one feature per page). 

- Why not all 3300 features are reported in the pdf? Are the features missing also normalized like the rest?

The exported Normalized txt table instead contains all 3300. Comparing values of features (found in the pdf report and not) between the Height and the Normalized txt tables, I would say all features have been normalized.

In addition, could you consider for future versions:

-In the pdf report, would be possible to order the features by their ID number?
-Would it be possible to normalize also the area (not only the peak height)?

Thank you!

Stefano


 

Re: normalization

Reply #1
As I told in the privately,
>>- Why not all 3300 features are reported in the pdf? Are the features missing also normalized like the rest?
Currently, the pdf exporter for "normalization check" works for reference matched metabolites in addition to top 100 unknown metabolite features having high abundant intensity.
It's to avoid a crash of PDF managing very very big size file.

>>-In the pdf report, would be possible to order the features by their ID number?
Sorry, I fixed it. Please wait a bit for the next update.:)

-Would it be possible to normalize also the area (not only the peak height)?
Yes, it should be future work.

Hiroshi

Re: normalization

Reply #2
Thanks a lot Hiroshi!