Skip to main content
Topic: MS/MS Spectra in mzML File (Read 166 times) previous topic - next topic

MS/MS Spectra in mzML File

Hi Hiroshi,

I am trying out a single +ve mode LC-MS/MS DDA data file (CE: 40 eV) acquired data for MS-DIAL processing.
Though, upon conversion to mzML using msConvert (oth 32 , 64 bit did not work out, peak picking all conditions did not help) can see lots of MS2 spectral data using Mass++ (See pic below) but can not see any MS/MS spectra in MS-DIAL interface (see pic). Attached are the parameters (.txt file) used too. Why is MS-DIAL not able to find the MS/MS data in my processing workflow ?

Not sure what am I doing wrong in MS-DIAL for this single data file ?

Thanks a lot for the help.

Biswa
Biswapriya B. Misra, PhD
Assistant Professor
Center for Precision Medicine
Department of Internal Medicine
Section on Molecular Medicine
Wake Forest University School of Medicine
Cell: +1-352-215-6040
Office: +1-336-713-4622
Profile: http://profiles.wakehealth.edu/display/Person/bmisra

Re: MS/MS Spectra in mzML File

Reply #1
do you have the right precursor mass in the MS2 spectra data? in your screenshot it looks like all the MS2 spectra are for m/z ~764 ion