identified compounds

December 24, 2020, 07:43:09 PM

Hi, everyone
I have a problem that I want to identified metabolites by MS-DIA, but I don't understand ion table in peek spot viewer and alignment spot viewer, which one should I choose.

Re: identified compounds

Reply #1 – December 27, 2020, 04:12:05 PM

Hi Dr. GAO,
It depends on if you are working on a particular sample or the alignment. If you double click any sample file in the "File navigator" then the software will directly switch to "peak spot viewer" where you can only see peak detected in this sample. If you choose any alignment in the "alignment navigator", then you will see all peaks aligned together across all samples in the "alignment spot viewer". when you click any spot in the "alignment spot viewer", you will see the intensity of this peak across all samples in "bar chart of aligned spot (NH)".
I hope my answer is helpful.

best,
Sukis